› 4o8p

X-ray diffraction
1.56Å resolution

Crystal structure of SthAraf62A, a GH62 family alpha-L-arabinofuranosidase from Streptomyces thermoviolaceus, bound to xylotetraose

Released:
DOI: 10.2210/pdb4o8p/pdb
PDB entry 4o8p coloured by chain, front view.
PDB entry 4o8p coloured by chain, side view.
PDB entry 4o8p coloured by chain, top view.
Source organism: Streptomyces thermoviolaceus
Primary publication:
Elucidation of the molecular basis for arabinoxylan-debranching activity of a thermostable family GH62 α-l-arabinofuranosidase from Streptomyces thermoviolaceus.
Wang W, Mai-Gisondi G, Stogios PJ, Kaur A, Xu X, Cui H, Turunen O, Savchenko A, Master ER
Appl Environ Microbiol 80 5317-29 (2014)
PMID: 24951792

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-565308 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
non-reducing end alpha-L-arabinofuranosidase Chain: A
Molecule details ›
Chain: A
Length: 384 amino acids
Theoretical weight: 42.1 KDa
Source organism: Streptomyces thermoviolaceus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q76BV4 (Residues: 1-363; Coverage: 100%)
Gene name: stxIV
Sequence domains: Glycosyl hydrolase family 62
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
3 bound ligands:
Ligand CA 1 x CA
Ligand PE3 2 x PE3
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 42.491Å b: 64.637Å c: 121.303Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.13 0.153
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

GH62 arabinofuranosidases: Structure, function and applications.
Wilkens et al. (2017)
2 more

1 mention without citation

Elucidation of the molecular basis for arabinoxylan-debranching activity of a thermostable family GH62 α-l-arabinofuranosidase from Streptomyces thermoviolaceus.
Wang et al. (2014)