› 4oga

X-ray diffraction
3.5Å resolution

Insulin in complex with Site 1 of the human insulin receptor

Released:
DOI: 10.2210/pdb4oga/pdb
PDB entry 4oga coloured by chain, front view.
PDB entry 4oga coloured by chain, side view.
PDB entry 4oga coloured by chain, top view.
Source organisms:
Primary publication:
Protective hinge in insulin opens to enable its receptor engagement.
Menting JG, Yang Y, Chan SJ, Phillips NB, Smith BJ, Whittaker J, Wickramasinghe NP, Whittaker LJ, Pandyarajan V, Wan ZL, Yadav SP, Carroll JM, Strokes N, Roberts CT, Ismail-Beigi F, Milewski W, Steiner DF, Chauhan VS, Ward CW, Weiss MA, Lawrence MC
Proc Natl Acad Sci U S A 111 E3395-404 (2014)
PMID: 25092300

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208398 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (8 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family
monoclonal antibody fab 83-7 fragment - heavy chain Chain: C
Molecule details ›
Chain: C
Length: 118 amino acids
Theoretical weight: 12.89 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
monoclonal antibody fab 83-7 fragment - light chain Chain: D
Molecule details ›
Chain: D
Length: 114 amino acids
Theoretical weight: 12.68 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Insulin receptor subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 317 amino acids
Theoretical weight: 36.23 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06213 (Residues: 28-337; Coverage: 23%)
Gene name: INSR
Sequence domains:
Structure domains:
Insulin receptor subunit alpha Chain: F
Molecule details ›
Chain: F
Length: 16 amino acids
Theoretical weight: 1.92 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06213 (Residues: 731-746; Coverage: 1%)
  • Best match: P06213-2 (Residues: 731-746)
Gene name: INSR

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P23
Unit cell:
a: 169.04Å b: 169.04Å c: 169.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.264 0.284
Expression systems:
  • Saccharomyces cerevisiae
  • Mus musculus
  • Cricetulus griseus
  • Not provided

Quick links

Citations

16 review citations

Insulin Receptor Isoforms in Physiology and Disease: An Updated View.
Belfiore et al. (2017)
15 more

27 mentions without citation

Biosynthesis, structure, and folding of the insulin precursor protein.
Liu et al. (2018)
26 more