› 4oj5

X-ray diffraction
1.8Å resolution

Crystal Structure of a Putative Tailspike Protein (TSP1, orf210) from Escherichia coli O157:H7 Bacteriohage CBA120

Released:
DOI: 10.2210/pdb4oj5/pdb
PDB entry 4oj5 coloured by chain, front view.
PDB entry 4oj5 coloured by chain, side view.
PDB entry 4oj5 coloured by chain, top view.
Source organism: Escherichia virus CBA120
Primary publication:
Crystal structure of ORF210 from E. coli O157:H1 phage CBA120 (TSP1), a putative tailspike protein.
Chen C, Bales P, Greenfield J, Heselpoth RD, Nelson DC, Herzberg O
OpenAccess logo PLoS One 9 e93156 (2014)
PMID: 24671238

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-515314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tail spike TSP1/Gp66 N-terminal domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 776 amino acids
Theoretical weight: 82.86 KDa
Source organism: Escherichia virus CBA120
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: G3M189 (Residues: 1-770; Coverage: 100%)
Gene name: orf210
Sequence domains: Tail spike TSP1/Gp66 receptor binding N-terminal domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 123.621Å b: 147.823Å c: 170.631Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.203
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins.
Dunne et al. (2021)
12 other articles

5 mentions without citation

Crystal structure of ORF210 from E. coli O157:H1 phage CBA120 (TSP1), a putative tailspike protein.
Chen et al. (2014)
4 more