› 4ojg

X-ray diffraction
1.38Å resolution

The crystal structure of V84D mutant of S. solfataricus acylphosphatase

Released:
DOI: 10.2210/pdb4ojg/pdb
PDB entry 4ojg coloured by chain, front view.
PDB entry 4ojg coloured by chain, side view.
PDB entry 4ojg coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Edge strand engineering prevents native-like aggregation in Sulfolobus solfataricus acylphosphatase.
de Rosa M, Bemporad F, Pellegrino S, Chiti F, Bolognesi M, Ricagno S
FEBS J 281 4072-84 (2014)
PMID: 24893801

Function and Biology Details

Reaction catalysed: 
An acylphosphate + H(2)O = a carboxylate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acylphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.67 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97ZL0 (Residues: 1-101; Coverage: 100%)
Gene names: SSO0887, acyP
Sequence domains: Acylphosphatase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 35.411Å b: 45.582Å c: 103.739Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.198
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Understanding and predicting protein misfolding and aggregation: Insights from proteomics.
Pallarès et al. (2016)
6 other articles

1 mention without citation

Edge strand engineering prevents native-like aggregation in Sulfolobus solfataricus acylphosphatase.
de Rosa et al. (2014)