› 4ojz

X-ray diffraction
1.9Å resolution

Crystal Structure of Alg17c Mutant Y258A Complexed with Alginate Trisaccharide

Released:
DOI: 10.2210/pdb4ojz/pdb
PDB entry 4ojz coloured by chain, front view.
PDB entry 4ojz coloured by chain, side view.
PDB entry 4ojz coloured by chain, top view.
Source organism: Saccharophagus degradans 2-40
Primary publication:
Structure of a PL17 family alginate lyase demonstrates functional similarities among exotype depolymerases.
Park D, Jagtap S, Nair SK
J Biol Chem 289 8645-55 (2014)
PMID: 24478312

Function and Biology Details

Reaction catalysed: 
Cleavage of 4-deoxy-alpha-L-erythro-hex-4-enopyranuronoside oligosaccharides into 4-deoxy-alpha-L-erythro-hex-4-enopyranuronate monosaccharides.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Exo-oligoalginate lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 736 amino acids
Theoretical weight: 81.58 KDa
Source organism: Saccharophagus degradans 2-40
Expression system: Escherichia coli
UniProt:
  • Canonical: Q21FJ0 (Residues: 1-736; Coverage: 100%)
Gene names: Sde_3284, alg17A, alg17C
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: LGU, MAV, BEM
Carbohydrate polymer
2 bound ligands:
Ligand ZN 2 x ZN
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 85.82Å b: 128.62Å c: 88.13Å
α: 90° β: 111.81° γ: 90°
R-values:
R R work R free
0.181 0.179 0.209
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications.
Zhu et al. (2015)
7 more

6 mentions without citation

Diversity of Three-Dimensional Structures and Catalytic Mechanisms of Alginate Lyases.
Xu et al. (2018)
5 more