4ooz

X-ray diffraction
2.6Å resolution

Crystal structure of beta-1,4-D-mannanase from Cryptopygus antarcticus in complex with mannopentaose

Released:

Function and Biology Details

Reaction catalysed:
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-110148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Mannan endo-1,4-beta-mannosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 42.7 KDa
Source organism: Cryptopygus antarcticus
Expression system: Escherichia coli
UniProt:
  • Canonical: B4XC07 (Residues: 1-382; Coverage: 100%)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA
Carbohydrate polymer : NEW Components: BMA
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 74.023Å b: 82.453Å c: 164.324Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.271
Expression system: Escherichia coli