› 4ooz

X-ray diffraction
2.6Å resolution

Crystal structure of beta-1,4-D-mannanase from Cryptopygus antarcticus in complex with mannopentaose

Released:
DOI: 10.2210/pdb4ooz/pdb
PDB entry 4ooz coloured by chain, front view.
PDB entry 4ooz coloured by chain, side view.
PDB entry 4ooz coloured by chain, top view.
Source organism: Cryptopygus antarcticus
Primary publication:
Structure-based investigation into the functional roles of the extended loop and substrate-recognition sites in an endo-β-1,4-D-mannanase from the Antarctic springtail, Cryptopygus antarcticus.
Kim MK, An YJ, Song JM, Jeong CS, Kang MH, Kwon KK, Lee YH, Cha SS
Proteins 82 3217-23 (2014)
PMID: 25082572

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Mannan endo-1,4-beta-mannosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 42.7 KDa
Source organism: Cryptopygus antarcticus
Expression system: Escherichia coli
UniProt:
  • Canonical: B4XC07 (Residues: 1-382; Coverage: 100%)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BMA
Carbohydrate polymer
1 bound ligand:
Ligand BMA 2 x BMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 74.023Å b: 82.453Å c: 164.324Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.271
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Evolution and functional characterization of CAZymes belonging to subfamily 10 of glycoside hydrolase family 5 (GH5_10) in two species of phytophagous beetles.
Busch et al. (2017)
3 more

2 mentions without citation

Structural Features of a Bacteroidetes-Affiliated Cellulase Linked with a Polysaccharide Utilization Locus.
Naas et al. (2015)
1 more