4oxp

X-ray diffraction
2.1Å resolution

X-ray crystal structure of the S1 and 5'-sensor domains of RNase E from Caulobacter crescentus

Released:
Model geometry
Fit model/data
Source organism: Caulobacter vibrioides CB15
Primary publication:
Molecular recognition of RhlB and RNase D in the Caulobacter crescentus RNA degradosome.
OpenAccess logo Nucleic Acids Res 42 13294-305 (2014)
PMID: 25389270

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease E Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.98 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9A749 (Residues: 1-270; Coverage: 30%)
Gene names: CC_1877, rne
Sequence domains: Ribonuclease E/G family
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P43212
Unit cell:
a: 62.53Å b: 62.53Å c: 155.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.254
Expression system: Escherichia coli