› 4pft

X-ray diffraction
1.75Å resolution

Crystal structure of mannobiose bound oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1223) from THERMOTOGA MARITIMA at 1.75 A resolution

Released:
DOI: 10.2210/pdb4pft/pdb
PDB entry 4pft coloured by chain, front view.
PDB entry 4pft coloured by chain, side view.
PDB entry 4pft coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Duplication of genes in an ATP-binding cassette transport system increases dynamic range while maintaining ligand specificity.
Ghimire-Rijal S, Lu X, Myles DA, Cuneo MJ
J Biol Chem 289 30090-100 (2014)
PMID: 25210043

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Solute-binding protein family 5 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 544 amino acids
Theoretical weight: 62.88 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X0V0 (Residues: 20-557; Coverage: 97%)
Gene name: TM_1223
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA
Carbohydrate polymer
2 bound ligands:
Ligand MG 2 x MG
Ligand NO3 3 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 96.489Å b: 53.54Å c: 106.28Å
α: 90° β: 110.47° γ: 90°
R-values:
R R work R free
0.154 0.152 0.204
Expression system: Escherichia coli

Quick links

Citations

1 review citation

ECF-Type ATP-Binding Cassette Transporters.
Rempel et al. (2019)
7 other articles

2 mentions without citation

Duplication of genes in an ATP-binding cassette transport system increases dynamic range while maintaining ligand specificity.
Ghimire-Rijal et al. (2014)
1 more