› 4pfu

X-ray diffraction
2.05Å resolution

Crystal structure of mannobiose bound oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1226) from THERMOTOGA MARITIMA at 2.05 A resolution

Released:
DOI: 10.2210/pdb4pfu/pdb
PDB entry 4pfu coloured by chain, front view.
PDB entry 4pfu coloured by chain, side view.
PDB entry 4pfu coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Duplication of genes in an ATP-binding cassette transport system increases dynamic range while maintaining ligand specificity.
Ghimire-Rijal S, Lu X, Myles DA, Cuneo MJ
J Biol Chem 289 30090-100 (2014)
PMID: 25210043

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
ABC transporter substrate-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 547 amino acids
Theoretical weight: 63.84 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA
Carbohydrate polymer
2 bound ligands:
Ligand MG 2 x MG
Ligand SO4 20 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2221
Unit cell:
a: 100.911Å b: 193.867Å c: 159.513Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.208
Expression system: Escherichia coli

Quick links

Citations

1 review citation

ECF-Type ATP-Binding Cassette Transporters.
Rempel et al. (2019)
7 other articles

1 mention without citation

Duplication of genes in an ATP-binding cassette transport system increases dynamic range while maintaining ligand specificity.
Ghimire-Rijal et al. (2014)