› 4pqb

X-ray diffraction
1.94Å resolution

A sperm whale myoglobin double mutant L29E/F43H Mb with a non-native bis-His (His64/His93) coordination

Released:
DOI: 10.2210/pdb4pqb/pdb
PDB entry 4pqb coloured by chain, front view.
PDB entry 4pqb coloured by chain, side view.
PDB entry 4pqb coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Regulating the coordination state of a heme protein by a designed distal hydrogen-bonding network.
Du JF, Li W, Li L, Wen GB, Lin YW, Tan X
OpenAccess logo ChemistryOpen 4 97-101 (2015)
PMID: 25969804

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.37 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli
UniProt:
  • Canonical: P02185 (Residues: 1-154; Coverage: 100%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 33.968Å b: 60.898Å c: 75.356Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.182 0.219
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Peroxidase Activity of Human Hemoproteins: Keeping the Fire under Control.
Vlasova II. (2018)
1 more

3 mentions without citation

Crystal structure of the antimicrobial peptidase lysostaphin from Staphylococcus simulans.
Sabala et al. (2014)
2 more