› 4q0m

X-ray diffraction
2.23Å resolution

Crystal structure of Pyrococcus furiosus L-asparaginase

Released:
DOI: 10.2210/pdb4q0m/pdb
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PDB entry 4q0m coloured by chain, front view.
PDB entry 4q0m coloured by chain, side view.
PDB entry 4q0m coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Structural and functional insights into an archaeal L-asparaginase obtained through the linker-less assembly of constituent domains.
Tomar R, Sharma P, Srivastava A, Bansal S, Kundu B
Acta Crystallogr D Biol Crystallogr 70 3187-97 (2014)
PMID: 25478837

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 35.93 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZE8 (Residues: 1-326; Coverage: 100%)
Gene name: PF2047
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand GOL 3 x GOL
Ligand 4CS 1 x 4CS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: R32
Unit cell:
a: 116.38Å b: 116.38Å c: 153.941Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.257
Expression system: Escherichia coli

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Citations

3 review citations

Structural and biophysical aspects of l-asparaginases: a growing family with amazing diversity.
Loch et al. (2021)
2 more

6 mentions without citation

The C-terminal region of the non-structural protein 2B from Hepatitis A Virus demonstrates lipid-specific viroporin-like activity.
Shukla et al. (2015)
5 more

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