› 4qb1

X-ray diffraction
2.19Å resolution

Structure of CBM35 from Paenibacillus barcinonensis

Released:
DOI: 10.2210/pdb4qb1/pdb
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PDB entry 4qb1 coloured by chain, front view.
PDB entry 4qb1 coloured by chain, side view.
PDB entry 4qb1 coloured by chain, top view.
Source organism: Paenibacillus barcinonensis
Primary publication:
Structural analysis of glucuronoxylan-specific Xyn30D and its attached CBM35 domain gives insights into the role of modularity in specificity.
Sainz-Polo MA, Valenzuela SV, González B, Pastor FI, Sanz-Aparicio J
J Biol Chem 289 31088-101 (2014)
PMID: 25202007

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CBM6 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 17.42 KDa
Source organism: Paenibacillus barcinonensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: H6WCZ0 (Residues: 422-562; Coverage: 27%)
Gene names: DFQ00_101274, xyn30d
Sequence domains: Carbohydrate binding module (family 6)
Structure domains: Galactose-binding domain-like

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Cu FINE FOCUS
Spacegroup: P212121
Unit cell:
a: 39.4Å b: 47.45Å c: 103.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.269
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Endo-xylanases as tools for production of substituted xylooligosaccharides with prebiotic properties.
Nordberg Karlsson et al. (2018)
4 more

3 mentions without citation

Identification and analysis of an early diagnostic marker for malignant melanoma: ZAR1 intra-genic differential methylation.
Shinojima et al. (2010)
2 more

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