› 4ql0

X-ray diffraction
2.5Å resolution

Crystal Structure Analysis of the Membrane Transporter FhaC (double mutant V169T, I176N)

Released:
DOI: 10.2210/pdb4ql0/pdb
PDB entry 4ql0 coloured by chain, front view.
PDB entry 4ql0 coloured by chain, side view.
PDB entry 4ql0 coloured by chain, top view.
Source organism: Bordetella pertussis Tohama I
Primary publication:
Conserved Omp85 lid-lock structure and substrate recognition in FhaC.
Maier T, Clantin B, Gruss F, Dewitte F, Delattre AS, Jacob-Dubuisson F, Hiller S, Villeret V
OpenAccess logo Nat Commun 6 7452 (2015)
PMID: 26058369

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Filamentous hemagglutinin transporter protein FhaC Chain: A
Molecule details ›
Chain: A
Length: 554 amino acids
Theoretical weight: 61.42 KDa
Source organism: Bordetella pertussis Tohama I
Expression system: Escherichia coli
UniProt:
  • Canonical: P35077 (Residues: 31-584; Coverage: 100%)
Gene names: BP1884, fhaC
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand PGE 1 x PGE
Ligand PEG 1 x PEG
Ligand BOG 3 x BOG
Ligand PO4 1 x PO4
Ligand 1PE 1 x 1PE
Ligand PG4 1 x PG4
Ligand P6G 1 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 106.38Å b: 136.95Å c: 110.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.217 0.258
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Biological Functions of the Secretome of Neisseria meningitidis.
Tommassen et al. (2017)
5 more

4 mentions without citation

Two-Partner Secretion: Combining Efficiency and Simplicity in the Secretion of Large Proteins for Bacteria-Host and Bacteria-Bacteria Interactions.
Guérin et al. (2017)
3 more