› 4qvh

X-ray diffraction
1.75Å resolution

Crystal structure of the essential Mycobacterium tuberculosis phosphopantetheinyl transferase PptT, solved as a fusion protein with maltose binding protein

Released:
DOI: 10.2210/pdb4qvh/pdb
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Source organisms:
Primary publication:
Crystal structure of the essential Mycobacterium tuberculosis phosphopantetheinyl transferase PptT, solved as a fusion protein with maltose binding protein.
Jung J, Bashiri G, Johnston JM, Brown AS, Ackerley DF, Baker EN
J Struct Biol 188 274-8 (2014)
PMID: 25450595

Function and Biology Details

Reaction catalysed: 
CoA-(4'-phosphopantetheine) + an apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + an [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
4'-phosphopantetheinyl transferase PptT; Maltose/maltodextrin-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 598 amino acids
Theoretical weight: 65.17 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: O33336 (Residues: 1-227; Coverage: 100%)
Gene names: JW3994, Rv2794c, b4034, malE, pptT
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
4 bound ligands:
Ligand COA 2 x COA
Ligand MG 2 x MG
Ligand GOL 7 x GOL
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 77.699Å b: 126.029Å c: 143.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.154 0.191
Expression system: Escherichia coli

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Citations

3 citation in other articles

In Vitro and In Vivo Inhibition of the Mycobacterium tuberculosis Phosphopantetheinyl Transferase PptT by Amidinoureas.
Ottavi et al. (2022)
2 more

3 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
2 more

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