PDB 4rq4 structure summary ‹ Protein Data Bank in Europe (PDBe)

Function and Biology Details

Reactions catalysed:

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

Biochemical function:

class I DNA-(apurinic or apyrimidinic site) endonuclease activity

Biological process:

DNA biosynthetic process

Cellular component:

protein-containing complex

Sequence domains:

4 more domains

Structure analysis Details

Assembly composition:

hetero tetramer (preferred)

Assembly name:

DNA polymerase beta and DNA (preferred)

PDBe Complex ID:

PDB-CPX-118133 (preferred)

Entry contents:

1 distinct polypeptide molecule
3 distinct DNA molecules

Macromolecules (4 distinct):

DNA polymerase beta Chain: A

Molecule details ›

Chain: A
Length: 343 amino acids
Theoretical weight: 39.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:

Canonical: P06746 (Residues: 1-335; Coverage: 100%)

Gene name: POLB
Sequence domains:

Structure domains:

DNA (5'-D(P*GP*TP*CP*GP*G)-3') Chain: D

Molecule details ›

Chain: D
Length: 5 nucleotides
Theoretical weight: 1.54 KDa
Source organism: synthetic construct
Expression system: Not provided

DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*CP*A)-3') Chain: P

Molecule details ›

Chain: P
Length: 11 nucleotides
Theoretical weight: 3.37 KDa
Source organism: synthetic construct
Expression system: Not provided

DNA (5'-D(*CP*CP*GP*AP*CP*(8OG)P*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3') Chain: T

Molecule details ›

Chain: T
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: synthetic construct
Expression system: Not provided