› 4ry8

X-ray diffraction
1.75Å resolution

Crystal structure of 5-methylthioribose transporter solute binding protein TLET_1677 from Thermotoga lettingae TMO TARGET EFI-511109 in complex with 5-methylthioribose

Released:
DOI: 10.2210/pdb4ry8/pdb
PDB entry 4ry8 coloured by chain, front view.
PDB entry 4ry8 coloured by chain, side view.
PDB entry 4ry8 coloured by chain, top view.
Source organism: Pseudothermotoga lettingae TMO
Entry authors: Patskovsky Y, Toro R, Bhosle R, Al obaidi N, Morisco LL, Wasserman SR, Chamala S, Attonito JD, Scott glenn A, Chowdhury S, Lafleur J, Hillerich B, Siede RD, Love J, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108632 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic binding protein domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 329 amino acids
Theoretical weight: 36.59 KDa
Source organism: Pseudothermotoga lettingae TMO
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8F7U7 (Residues: 22-348; Coverage: 100%)
Gene name: Tlet_1677
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SR1 4 x SR1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 57.622Å b: 67.848Å c: 83.653Å
α: 91.83° β: 92.39° γ: 89.93°
R-values:
R R work R free
0.216 0.214 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links