› 4rzc

X-ray diffraction
2.72Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213616 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fv M6P-1 light chain Chains: A, C, E, H
Molecule details ›
Chains: A, C, E, H
Length: 119 amino acids
Theoretical weight: 12.65 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fv M6P-1 heavy chain Chains: B, D, F, L
Molecule details ›
Chains: B, D, F, L
Length: 112 amino acids
Theoretical weight: 12.08 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand M6P 4 x M6P
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 60.607Å b: 128.05Å c: 127.295Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.267
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Natural and Synthetic Sialylated Glycan Microarrays and Their Applications.
McQuillan et al. (2019)
1 more

1 mention without citation

Single-chain antibody-fragment M6P-1 possesses a mannose 6-phosphate monosaccharide-specific binding pocket that distinguishes N-glycan phosphorylation in a branch-specific manner†.
Blackler et al. (2016)