› 4u5e

X-ray diffraction
3.51Å resolution

Crystal structure of GluA2 T625G, con-ikot-ikot snail toxin, partial agonist KA and postitive modulator (R,R)-2b complex

Released:
DOI: 10.2210/pdb4u5e/pdb
PDB entry 4u5e coloured by chain, front view.
PDB entry 4u5e coloured by chain, side view.
PDB entry 4u5e coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism.
Chen L, Dürr KL, Gouaux E
Science 345 1021-6 (2014)
PMID: 25103405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-533671 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glutamate receptor 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 814 amino acids
Theoretical weight: 91.34 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
UniProt:
  • Canonical: P19491 (Residues: 25-845; Coverage: 94%)
  • Best match: P19491-2 (Residues: 25-845)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Con-ikot-ikot Chains: E, F
Molecule details ›
Chains: E, F
Length: 90 amino acids
Theoretical weight: 9.75 KDa
Source organism: Conus striatus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CB20 (Residues: 38-123; Coverage: 82%)
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

3 bound ligands:
Ligand KAI 4 x KAI
Ligand NAG 4 x NAG
Ligand FWF 2 x FWF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21212
Unit cell:
a: 161.27Å b: 366.69Å c: 109.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.24 0.276
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

22 review citations

Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.
Hansen et al. (2021)
21 more

2 mentions without citation

Emerging structural insights into the function of ionotropic glutamate receptors.
Karakas et al. (2015)
1 more