› 4uni

X-ray diffraction
2.6Å resolution

beta-(1,6)-galactosidase from Bifidobacterium animalis subsp. lactis Bl-04 in complex with galactose

Released:
DOI: 10.2210/pdb4uni/pdb
PDB entry 4uni coloured by chain, front view.
PDB entry 4uni coloured by chain, side view.
PDB entry 4uni coloured by chain, top view.
Source organism: Bifidobacterium animalis subsp. lactis Bl-04
Primary publication:
A β1-6/β1-3 galactosidase from Bifidobacterium animalis subsp. lactis Bl-04 gives insight into sub-specificities of β-galactoside catabolism within Bifidobacterium.
Viborg AH, Fredslund F, Katayama T, Nielsen SK, Svensson B, Kitaoka M, Lo Leggio L, Abou Hachem M
Mol Microbiol (2014)
PMID: 25287704

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BETA-GALACTOSIDASE Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 695 amino acids
Theoretical weight: 78.33 KDa
Source organism: Bifidobacterium animalis subsp. lactis Bl-04
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand GAL 3 x GAL
Ligand PGE 6 x PGE
Ligand PEG 6 x PEG
Ligand GOL 1 x GOL
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: C2221
Unit cell:
a: 139.52Å b: 199.44Å c: 217.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Cold-Active β-Galactosidases: Insight into Cold Adaption Mechanisms and Biotechnological Exploitation.
Mangiagalli et al. (2021)
11 other articles

6 mentions without citation

Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes.
Fushinobu et al. (2021)
5 more