› 4uso

X-ray diffraction
1.95Å resolution

X-ray structure of the CCL2 lectin in complex with sialyl lewis X

Released:
DOI: 10.2210/pdb4uso/pdb
PDB entry 4uso coloured by chain, front view.
PDB entry 4uso coloured by chain, side view.
PDB entry 4uso coloured by chain, top view.
Source organism: Coprinopsis cinerea
Primary publication:
Dimerization of the fungal defense lectin CCL2 is essential for its toxicity against nematodes.
Bleuler-Martinez S, Stutz K, Sieber R, Collot M, Mallet JM, Hengartner M, Schubert M, Varrot A, Künzler M
Glycobiology 27 486-500 (2017)
PMID: 27980000

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-502648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
CCL2-like lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 153 amino acids
Theoretical weight: 16.6 KDa
Source organism: Coprinopsis cinerea
Expression system: Escherichia coli
UniProt:
  • Canonical: B3GA02 (Residues: 2-142; Coverage: 99%)
Gene name: ccl2
Sequence domains: CCL2 lectin-like
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, SIA, FUC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6122
Unit cell:
a: 121.68Å b: 121.68Å c: 144.907Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.204
Expression system: Escherichia coli

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Citations

1 review citation

CNL-Clitocybe nebularis Lectin-The Fungal GalNAcβ1-4GlcNAc-Binding Lectin.
Sabotič et al. (2019)
10 other articles