› 4uyp

X-ray diffraction
1.49Å resolution

High resolution structure of the third cohesin ScaC in complex with the ScaB dockerin with a mutation in the N-terminal helix (IN to SI) from Acetivibrio cellulolyticus displaying a type I interaction.

Released:
DOI: 10.2210/pdb4uyp/pdb
PDB entry 4uyp coloured by chain, front view.
PDB entry 4uyp coloured by chain, side view.
PDB entry 4uyp coloured by chain, top view.
Source organism: Acetivibrio cellulolyticus
Primary publication:
Cell-surface Attachment of Bacterial Multienzyme Complexes Involves Highly Dynamic Protein-Protein Anchors.
Cameron K, Najmudin S, Alves VD, Bayer EA, Smith SP, Bule P, Waller H, Ferreira LM, Gilbert HJ, Fontes CM
J Biol Chem 290 13578-90 (2015)
PMID: 25855788

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182159 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SLH domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 151 amino acids
Theoretical weight: 16.51 KDa
Source organism: Acetivibrio cellulolyticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WYN2 (Residues: 326-467; Coverage: 12%)
Gene name: scaC
Sequence domains: Cohesin domain
Structure domains: Immunoglobulin-like
Dockerin domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 75 amino acids
Theoretical weight: 8.38 KDa
Source organism: Acetivibrio cellulolyticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WYN3 (Residues: 868-942; Coverage: 8%)
Gene name: scaB
Sequence domains: Dockerin type I domain
Structure domains: Dockerin domain

Ligands and Environments

4 bound ligands:
Ligand SO4 13 x SO4
Ligand EPE 3 x EPE
Ligand MPD 6 x MPD
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P41212
Unit cell:
a: 107.75Å b: 107.75Å c: 100.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Single versus dual-binding conformations in cellulosomal cohesin-dockerin complexes.
Nash et al. (2016)
2 more

7 mentions without citation

Broad phylogeny and functionality of cellulosomal components in the bovine rumen microbiome.
Bensoussan et al. (2017)
6 more