› 4uzm

Solution NMR

Shotgun proteolysis: A practical application

Released:
DOI: 10.2210/pdb4uzm/pdb
PDB entry 4uzm coloured by chain, ensemble of 20 models, front view.
PDB entry 4uzm coloured by chain, ensemble of 20 models, side view.
PDB entry 4uzm coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli
Primary publication:
Solution structure of a soluble fragment derived from a membrane protein by shotgun proteolysis.
Allen MD, Christie M, Jones P, Porebski BT, Roome B, Freund SM, Buckle AM, Bycroft M, Christ D
OpenAccess logo Protein Eng Des Sel 28 445-50 (2015)
PMID: 25877662

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-545718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative membrane protein IgaA homolog Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.44 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P45800 (Residues: 36-154; Coverage: 17%)
Gene names: JW3361, b3398, yrfF

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: CNS
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Complex Rcs Regulatory Cascade.
Wall et al. (2018)
1 other article

3 mentions without citation

In Silico Prediction and Prioritization of Novel Selective Antimicrobial Drug Targets in Escherichia coli.
Svanberg Frisinger et al. (2021)
2 more