› 4x04

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM CITROBACTER KOSERI (CKO_04899, TARGET EFI-510094) WITH BOUND D-glucuronate

Released:
DOI: 10.2210/pdb4x04/pdb
PDB entry 4x04 coloured by chain, front view.
PDB entry 4x04 coloured by chain, side view.
PDB entry 4x04 coloured by chain, top view.
Source organism: Citrobacter koseri ATCC BAA-895
Entry authors: Yadava U, Vetting MW, Al Obaidi NF, Toro R, Morisco LL, Benach J, Wasserman SR, Attonito JD, Scott Glenn A, Chamala S, Chowdhury S, Lafleur J, Love J, Seidel RD, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-501381 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,3-diketo-L-gulonate TRAP transporter substrate-binding periplasmic protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 323 amino acids
Theoretical weight: 36.77 KDa
Source organism: Citrobacter koseri ATCC BAA-895
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8AR30 (Residues: 28-327; Coverage: 100%)
Gene name: CKO_04899
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

3 bound ligands:
Ligand BDP 4 x BDP
Ligand CL 6 x CL
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 64 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P41212
Unit cell:
a: 175Å b: 175Å c: 125.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.205
Expression system: Escherichia coli BL21(DE3)

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