› 4xp9

X-ray diffraction
2.8Å resolution

X-ray structure of Drosophila dopamine transporter bound to psychostimulant D-amphetamine

Released:
DOI: 10.2210/pdb4xp9/pdb
PDB entry 4xp9 coloured by chain, front view.
PDB entry 4xp9 coloured by chain, side view.
PDB entry 4xp9 coloured by chain, top view.
Source organisms:
Primary publication:
Neurotransmitter and psychostimulant recognition by the dopamine transporter.
Wang KH, Penmatsa A, Gouaux E
Nature 521 322-7 (2015)
PMID: 25970245

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-565692 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Sodium-dependent dopamine transporter Chain: C
Molecule details ›
Chain: C
Length: 541 amino acids
Theoretical weight: 60.73 KDa
Source organism: Drosophila melanogaster
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7K4Y6 (Residues: 25-601; Coverage: 85%)
Gene names: CG8380, DAT, fmn
Sequence domains: Sodium:neurotransmitter symporter family
ANTIBODY FRAGMENT HEAVY CHAIN-PROTEIN, 9D5-HEAVY CHAIN Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.19 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
ANTIBODY FRAGMENT LIGHT CHAIN-PROTEIN, 9D5-LIGHT CHAIN Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
8 bound ligands:
Ligand Y01 1 x Y01
Ligand NAG 1 x NAG
Ligand MPO 1 x MPO
Ligand CLR 1 x CLR
Ligand 1WE 1 x 1WE
Ligand P4G 2 x P4G
Ligand NA 2 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 97.348Å b: 140.748Å c: 166.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.242
Expression systems:
  • Homo sapiens
  • Mus musculus

Quick links

Citations

43 review citations

Emerging Diversity in Lipid-Protein Interactions.
Corradi et al. (2019)
42 more

22 mentions without citation

New tricks for old dogs: improving the accuracy of biomolecular force fields by pair-specific corrections to non-bonded interactions.
Yoo et al. (2018)
21 more