› 4xvs

X-ray diffraction
1.9Å resolution

Crystal structure of HIV-1 donor 45 d45-01dG5 coreE gp120 with antibody 45-VRC01.H01+07.O-863513/45-VRC01.L01+07.O-110653 (VRC07_1995)

Released:
DOI: 10.2210/pdb4xvs/pdb
PDB entry 4xvs coloured by chain, front view.
PDB entry 4xvs coloured by chain, side view.
PDB entry 4xvs coloured by chain, top view.
Source organisms:
Primary publication:
Maturation and Diversity of the VRC01-Antibody Lineage over 15 Years of Chronic HIV-1 Infection.
Wu X, Zhang Z, Schramm CA, Joyce MG, Kwon YD, Zhou T, Sheng Z, Zhang B, O'Dell S, McKee K, Georgiev IS, Chuang GY, Longo NS, Lynch RM, Saunders KO, Soto C, Srivatsan S, Yang Y, Bailer RT, Louder MK, Mullikin JC, Connors M, Kwong PD, Mascola JR, Shapiro L
Cell 161 470-485 (2015)
PMID: 25865483

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229722 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
VRC07_1995 45-VRC01.H01+07.O-863513/45-VRC01.L01+07.O-110653 Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 228 amino acids
Theoretical weight: 24.74 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
45-VRC01.H01+07.O-863513/45-VRC01.L01+07.O-110653 Light chain Chain: L
Molecule details ›
Chain: L
Length: 210 amino acids
Theoretical weight: 23.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Envelope glycoprotein gp160 Chain: G
Molecule details ›
Chain: G
Length: 375 amino acids
Theoretical weight: 41.87 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: H9DP89 (Residues: 43-490; Coverage: 42%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein

Ligands and Environments

1 bound ligand:
Ligand NAG 8 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 67.641Å b: 69.334Å c: 200.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.214
Expression system: Homo sapiens

Quick links

Citations

37 review citations

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design.
Burton et al. (2016)
36 more

3 mentions without citation

Maturation and Diversity of the VRC01-Antibody Lineage over 15 Years of Chronic HIV-1 Infection.
Wu et al. (2015)
2 more