› 4xx6

X-ray diffraction
1.95Å resolution

Crystal structure of a glycosylated endo-beta-1,4-xylanase (glycoside hydrolase family 10/GH10) enzyme from Gloeophyllum trabeum

Released:
DOI: 10.2210/pdb4xx6/pdb
PDB entry 4xx6 coloured by chain, front view.
PDB entry 4xx6 coloured by chain, side view.
PDB entry 4xx6 coloured by chain, top view.
Source organism: Gloeophyllum trabeum ATCC 11539
Entry authors: Stogios PJ, Nocek B, Xu X, Cui H, Lowden M, Savchenko A

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (7 distinct):
Beta-xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 35.7 KDa
Source organism: Gloeophyllum trabeum ATCC 11539
Expression system: Aspergillus niger
UniProt:
  • Canonical: S7Q6I2 (Residues: 27-347; Coverage: 98%)
Gene name: GLOTRDRAFT_138785
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
7 bound ligands:
Ligand UNX 2 x UNX
Ligand NAG 3 x NAG
Ligand MG 2 x MG
Ligand PEG 3 x PEG
Ligand P6G 2 x P6G
Ligand GOL 7 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 52.414Å b: 99.345Å c: 147.267Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.204
Expression system: Aspergillus niger

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