› 4y9t

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF AN ABC TRANSPORTER SOLUTE BINDING PROTEIN (IPR025997) FROM AGROBACTERIUM VITIS S4 (Avi_5305, TARGET EFI-511224) WITH BOUND ALPHA-D-GLUCOSAMINE

Released:
DOI: 10.2210/pdb4y9t/pdb
PDB entry 4y9t coloured by chain, front view.
PDB entry 4y9t coloured by chain, side view.
PDB entry 4y9t coloured by chain, top view.
Source organism: Agrobacterium vitis S4
Primary publication:
Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine.
Yadava U, Vetting MW, Al Obaidi N, Carter MS, Gerlt JA, Almo SC
Acta Crystallogr F Struct Biol Commun 72 467-72 (2016)
PMID: 27303900

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110841 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic binding protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 346 amino acids
Theoretical weight: 37.43 KDa
Source organism: Agrobacterium vitis S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9K0Q5 (Residues: 24-346; Coverage: 100%)
Gene name: Avi_5305
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PA1 1 x PA1
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 36.634Å b: 63.611Å c: 127.394Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.189
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

In-silico molecular designs to treat neurologic and ophthalmologic diseases caused by sorbitol excess: engineering the Agrobacterium vitis protein.
Sharma et al. (2023)
1 more

1 mention without citation

Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine.
Yadava et al. (2016)