› 4yij

X-ray diffraction
1.64Å resolution

Crystal structure of Staphylcoccal nuclease variant Delta+PHS A109E at cryogenic temperature

Released:
DOI: 10.2210/pdb4yij/pdb
PDB entry 4yij coloured by chain, front view.
PDB entry 4yij coloured by chain, side view.
PDB entry 4yij coloured by chain, top view.
Source organism: Staphylococcus aureus
Entry authors: Skerritt LA, Bell-Upp PC, Schlessman JL, Garcia-Moreno EB

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-523735 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chains: A, B
Molecule details ›
Chains: A, B
Length: 143 amino acids
Theoretical weight: 16.2 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 70%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand THP 2 x THP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P212121
Unit cell:
a: 46.96Å b: 54.283Å c: 108.533Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links