› 4ys6

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF AN ABC TRANSPORTER SOLUTE BINDING PROTEIN (IPR025997) FROM CLOSTRIDIUM PHYTOFERMENTANS (Cphy_1585, TARGET EFI-511156) WITH BOUND BETA-D-GLUCOSE

Released:
DOI: 10.2210/pdb4ys6/pdb
PDB entry 4ys6 coloured by chain, front view.
PDB entry 4ys6 coloured by chain, side view.
PDB entry 4ys6 coloured by chain, top view.
Source organism: Lachnoclostridium phytofermentans ISDg
Entry authors: Vetting MW, Patskovsky Y, Al Obaidi NF, Toro R, Morisco LL, Benach J, Koss J, Wasserman SR, Attonito JD, Scott Glenn A, Chamala S, Chowdhury S, Lafleur J, Love J, Seidel RD, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic binding protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 39.65 KDa
Source organism: Lachnoclostridium phytofermentans ISDg
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A9KQP6 (Residues: 19-355; Coverage: 100%)
Gene name: Cphy_1585
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand BGC 1 x BGC
Ligand NA 5 x NA
1 modified residue:
Ligand MSE 13 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P6522
Unit cell:
a: 80.686Å b: 80.686Å c: 170.004Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.15 0.15 0.182
Expression system: Escherichia coli BL21(DE3)

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