› 4z2n

X-ray diffraction
1.92Å resolution

Crystal structure of human FACT SPT16 middle domain

Released:
DOI: 10.2210/pdb4z2n/pdb
PDB entry 4z2n coloured by chain, front view.
PDB entry 4z2n coloured by chain, side view.
PDB entry 4z2n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Integrated molecular mechanism directing nucleosome reorganization by human FACT.
Tsunaka Y, Fujiwara Y, Oyama T, Hirose S, Morikawa K
OpenAccess logo Genes Dev 30 673-86 (2016)
PMID: 26966247

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-578877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FACT complex subunit SPT16 Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 33.37 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q9Y5B9 (Residues: 644-930; Coverage: 27%)
Gene names: FACT140, FACTP140, SUPT16H
Sequence domains: Histone chaperone Rttp106-like, middle domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 160.891Å b: 49.748Å c: 40.048Å
α: 90° β: 95.18° γ: 90°
R-values:
R R work R free
0.178 0.174 0.216
Expression system: Baculovirus expression vector pFastBac1-HM

Quick links

Citations

24 review citations

Histone chaperone networks shaping chromatin function.
Hammond et al. (2017)
23 more

2 mentions without citation

Structure-function relationship of H2A-H2B specific plant histone chaperones.
Kumar et al. (2020)
1 more