› 4z9s

X-ray diffraction
2.3Å resolution

Non-covalent assembly of monoubiquitin that mimics K11 poly-ubiquitin

Released:
DOI: 10.2210/pdb4z9s/pdb
PDB entry 4z9s coloured by chain, front view.
PDB entry 4z9s coloured by chain, side view.
PDB entry 4z9s coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Tetrameric Assembly of Monoubiquitin Accurately Mimics the Lys11 Polyubiquitin Chain Structure.
Levin-Kravets O, Shohat N, Prag G
Biochemistry 54 4704-10 (2015)
PMID: 26171660

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62992 (Residues: 1-76; Coverage: 49%)
Gene names: RPS27A, UBA80, UBCEP1
Sequence domains: Ubiquitin family

Ligands and Environments

2 bound ligands:
Ligand MLA 6 x MLA
Ligand SCN 9 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 81.319Å b: 81.301Å c: 58.597Å
α: 90° β: 129.33° γ: 90°
R-values:
R R work R free
0.186 0.186 0.223
Expression system: Escherichia coli

Quick links

Citations

2 mentions without citation

Ion Activation Methods for Peptides and Proteins.
Macias et al. (2020)
1 more