› 4zh5

X-ray diffraction
1.35Å resolution

Crystal structure of Endoglucanase from Perinereis brevicirris with Cellobiose

Released:
DOI: 10.2210/pdb4zh5/pdb
PDB entry 4zh5 coloured by chain, front view.
PDB entry 4zh5 coloured by chain, side view.
PDB entry 4zh5 coloured by chain, top view.
Source organism: Perinereis brevicirris
Entry authors: Fewings RS, Swiderska A, Sanchez-Weatherby J, Sorensen T-M, Schnorr KM, Kneale GG, McGeehan JE

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-514415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 426 amino acids
Theoretical weight: 46.81 KDa
Source organism: Perinereis brevicirris
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: F2Z7L1 (Residues: 19-444; Coverage: 100%)
Gene name: pnbEG
Sequence domains: Glycosyl hydrolase family 9
Structure domains: Glycosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand NA 5 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 76.15Å b: 108.17Å c: 112.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 0.115 0.148
Expression system: Aspergillus oryzae

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