› 4zoc

X-ray diffraction
1.79Å resolution

Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorotriose

Released:
DOI: 10.2210/pdb4zoc/pdb
PDB entry 4zoc coloured by chain, front view.
PDB entry 4zoc coloured by chain, side view.
PDB entry 4zoc coloured by chain, top view.
Source organism: Listeria innocua Clip11262
Primary publication:
Functional and Structural Analysis of a β-Glucosidase Involved in β-1,2-Glucan Metabolism in Listeria innocua.
Nakajima M, Yoshida R, Miyanaga A, Abe K, Takahashi Y, Sugimoto N, Toyoizumi H, Nakai H, Kitaoka M, Taguchi H
OpenAccess logo PLoS One 11 e0148870 (2016)
PMID: 26886583

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 731 amino acids
Theoretical weight: 80.57 KDa
Source organism: Listeria innocua Clip11262
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92AS9 (Residues: 1-723; Coverage: 100%)
Gene name: lin1840
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand MG 2 x MG
Ligand BGC 2 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Unit cell:
a: 89.62Å b: 94.86Å c: 215.065Å
α: 90° β: 96.33° γ: 90°
R-values:
R R work R free
0.144 0.141 0.196
Expression system: Escherichia coli

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Citations

2 review citations

A glucotolerant β-glucosidase from the fungus Talaromyces amestolkiae and its conversion into a glycosynthase for glycosylation of phenolic compounds.
Méndez-Líter et al. (2020)
1 more