Function and Biology Details
Reactions catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
(1a) S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-monoubiquitinyl-[(E3-independent) ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Ubiquitin-conjugating enzyme E2, catalytic core domain
- Zinc finger, RING-type
- SH2 domain superfamily
- Adaptor protein Cbl, SH2-like domain
- Adaptor protein Cbl, N-terminal domain superfamily
- Ubiquitin-conjugating enzyme, active site
- Ubiquitin-conjugating enzyme/RWD-like
- Zinc finger, RING-type, conserved site
7 more domains
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-149758 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
E3 ubiquitin-protein ligase CBL
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 45.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 391 amino acids
Theoretical weight: 45.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P22681 (Residues: 47-435; Coverage: 43%)
Sequence domains:
- CBL proto-oncogene N-terminal domain 1
- CBL proto-oncogene N-terminus, EF hand-like domain
- CBL proto-oncogene N-terminus, SH2-like domain
- Zinc finger, C3HC4 type (RING finger)
Tyrosine-protein kinase ZAP-70
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.34 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
Length: 12 amino acids
Theoretical weight: 1.34 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
- Canonical: P43403 (Residues: 286-297; Coverage: 2%)
Ubiquitin-conjugating enzyme E2 D2
