› 4a8h

X-ray diffraction
2.5Å resolution

Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N-(phosphonoacetyl)-putrescine

Released:
DOI: 10.2210/pdb4a8h/pdb
PDB entry 4a8h coloured by chain, front view.
PDB entry 4a8h coloured by chain, side view.
PDB entry 4a8h coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
New insight into the transcarbamylase family: the structure of putrescine transcarbamylase, a key catalyst for fermentative utilization of agmatine.
Polo LM, Gil-Ortiz F, Cantín A, Rubio V
OpenAccess logo PLoS One 7 e31528 (2012)
PMID: 22363663

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182851 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putrescine carbamoyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 355 amino acids
Theoretical weight: 40.14 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q837U7 (Residues: 1-339; Coverage: 100%)
Gene names: EF_0732, agcB, argF-2, ptcA
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase

Ligands and Environments

5 bound ligands:
Ligand PUW 2 x PUW
Ligand TRS 2 x TRS
Ligand SO4 2 x SO4
Ligand EDO 3 x EDO
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P6322
Unit cell:
a: 117.2Å b: 117.2Å c: 225.345Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Sources and Fates of Carbamyl Phosphate: A Labile Energy-Rich Molecule with Multiple Facets.
Shi et al. (2018)
1 more

1 mention without citation

New insight into the transcarbamylase family: the structure of putrescine transcarbamylase, a key catalyst for fermentative utilization of agmatine.
Polo et al. (2012)