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The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex.

Murachelli AG, Ebert J, Basquin C, Le Hir H, Conti E
Nat Struct Mol Biol 19 378-86 (2012)
Related entries: 4a6q, 4a8x

Cited: 56 times
EuropePMC logo PMID: 22388736

Abstract

The ASAP complex interacts with the exon-junction complex (EJC), a messenger ribonucleoprotein complex involved in post-transcriptional regulation. The three ASAP subunits (Acinus, RNPS1 and SAP18) have been individually implicated in transcriptional regulation, pre-mRNA splicing and mRNA quality control. To shed light on the basis for and consequences of ASAP's interaction with the EJC, we have determined the 1.9-Å resolution structure of a eukaryotic ASAP core complex. The RNA-recognition motif of RNPS1 binds to a conserved motif of Acinus with a recognition mode similar to that observed in splicing U2AF proteins. The Acinus-RNPS1 platform recruits the ubiquitin-like domain of SAP18, forming a ternary complex that has both RNA- and protein-binding properties. Unexpectedly, our structural analysis identified an Acinus-like motif in Pinin, another EJC-associated splicing factor. We show that Pinin physically interacts with RNPS1 and SAP18, forming an alternative ternary complex, PSAP.

Reviews citing this publication (10)

  1. The exon junction complex as a node of post-transcriptional networks. Le Hir H, Saulière J, Wang Z. Nat Rev Mol Cell Biol 17 41-54 (2016)
  2. A Day in the Life of the Exon Junction Complex. Schlautmann LP, Gehring NH. Biomolecules 10 E866 (2020)
  3. Nonsense-mediated mRNA decay at the crossroads of many cellular pathways. Lejeune F. BMB Rep 50 175-185 (2017)
  4. Multifaceted Regulation of Gene Expression by the Apoptosis- and Splicing-Associated Protein Complex and Its Components. Deka B, Singh KK. Int J Biol Sci 13 545-560 (2017)
  5. PAX6 Alternative Splicing and Corneal Development. Park JW, Yang J, Xu RH. Stem Cells Dev 27 367-377 (2018)
  6. The Physiological Roles of the Exon Junction Complex in Development and Diseases. Asthana S, Martin H, Rupkey J, Patel S, Yoon J, Keegan A, Mao Y. Cells 11 1192 (2022)
  7. The exon junction complex: structural insights into a faithful companion of mammalian mRNPs. Gerbracht JV, Gehring NH. Biochem Soc Trans 46 153-161 (2018)
  8. No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors. Mailliot J, Vivoli-Vega M, Schaffitzel C. Biochem J 479 973-993 (2022)
  9. Alternative splicing in ABA signaling during seed germination. Sybilska E, Daszkowska-Golec A. Front Plant Sci 14 1144990 (2023)
  10. Diverse Roles of the Exon Junction Complex Factors in the Cell Cycle, Cancer, and Neurodevelopmental Disorders-Potential for Therapeutic Targeting. Martin H, Rupkey J, Asthana S, Yoon J, Patel S, Mott J, Pei Z, Mao Y. Int J Mol Sci 23 10375 (2022)

Articles citing this publication (46)



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