› 4ady

X-ray diffraction
2.7Å resolution

Crystal structure of 26S proteasome subunit Rpn2

Released:
DOI: 10.2210/pdb4ady/pdb
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PDB entry 4ady coloured by chain, front view.
PDB entry 4ady coloured by chain, side view.
PDB entry 4ady coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The structure of the 26S proteasome subunit Rpn2 reveals its PC repeat domain as a closed toroid of two concentric α-helical rings.
He J, Kulkarni K, da Fonseca PC, Krutauz D, Glickman MH, Barford D, Morris EP
Structure 20 513-21 (2012)
PMID: 22405010

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152363 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
26S proteasome regulatory subunit RPN2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 963 amino acids
Theoretical weight: 107.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P32565 (Residues: 2-945; Coverage: 100%)
Gene names: RPN2, SEN3, YIL075C
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 42 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 73.96Å b: 148.66Å c: 114.03Å
α: 90° β: 107.39° γ: 90°
R-values:
R R work R free
0.184 0.183 0.24
Expression system: Escherichia coli BL21

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Citations

16 review citations

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
15 more

12 mentions without citation

Molecular architecture and assembly of the eukaryotic proteasome.
Tomko et al. (2013)
11 more

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