› 4ae9

X-ray diffraction
2.3Å resolution

Structure and function of the Human Sperm-Specific Isoform of Protein Kinase A (PKA) Catalytic Subunit C alpha 2

Released:
DOI: 10.2210/pdb4ae9/pdb
PDB entry 4ae9 coloured by chain, front view.
PDB entry 4ae9 coloured by chain, side view.
PDB entry 4ae9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and function of the human sperm-specific isoform of protein kinase A (PKA) catalytic subunit Cα2.
Hereng TH, Backe PH, Kahmann J, Scheich C, Bjørås M, Skålhegg BS, Rosendal KR
J Struct Biol 178 300-10 (2012)
PMID: 22504716

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase catalytic subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 40.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P17612 (Residues: 10-351; Coverage: 97%)
  • Best match: P17612-2 (Residues: 1-343)
Gene names: PKACA, PRKACA
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand SEP 4 x SEP
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 76.396Å b: 89.971Å c: 115.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.264
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Sperm proteomics: road to male fertility and contraception.
Rahman et al. (2013)
4 other articles

3 mentions without citation

The Molecular Basis for Specificity at the Level of the Protein Kinase a Catalytic Subunit.
Søberg et al. (2018)
2 more