› 4ar7

X-ray diffraction
1.23Å resolution

X-ray structure of the cyan fluorescent protein mTurquoise

Released:
DOI: 10.2210/pdb4ar7/pdb
PDB entry 4ar7 coloured by chain, front view.
PDB entry 4ar7 coloured by chain, side view.
PDB entry 4ar7 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K.
von Stetten D, Noirclerc-Savoye M, Goedhart J, Gadella TW, Royant A
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 878-82 (2012)
PMID: 22869113

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.72 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SWG 1 x SWG

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 51.452Å b: 62.558Å c: 69.543Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.143 0.166
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Spatial Intensity Distribution Analysis: Studies of G Protein-Coupled Receptor Oligomerisation.
Pediani et al. (2018)
1 more

3 mentions without citation

Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K.
von Stetten et al. (2012)
2 more