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Molecular basis for specific regulation of neuronal kinesin-3 motors by doublecortin family proteins.

<div class='caption-title'>Kif1a Is Mislocalized in Dcx/Dclk1-Deficient Neurons</div>
<div class='caption-title'>Vamp2 Transport from the Cell Body into Neurites Is Dependent on Kif1a</div>
<div class='caption-title'>Efflux of Vamp2-GFP into Neurites Is Impaired in Dcx/Dclk1-Deficient Neurons</div>
Liu JS, Schubert CR, Fu X, Fourniol FJ, Jaiswal JK, Houdusse A, Stultz CM, Moores CA, Walsh CA
OpenAccess logo Mol Cell 47 707-21 (2012)
Related entries: 2xrp, 4atu

Cited: 80 times
EuropePMC logo PMID: 22857951

Abstract

Doublecortin (Dcx) defines a growing family of microtubule (MT)-associated proteins (MAPs) involved in neuronal migration and process outgrowth. We show that Dcx is essential for the function of Kif1a, a kinesin-3 motor protein that traffics synaptic vesicles. Neurons lacking Dcx and/or its structurally conserved paralogue, doublecortin-like kinase 1 (Dclk1), show impaired Kif1a-mediated transport of Vamp2, a cargo of Kif1a, with decreased run length. Human disease-associated mutations in Dcx's linker sequence (e.g., W146C, K174E) alter Kif1a/Vamp2 transport by disrupting Dcx/Kif1a interactions without affecting Dcx MT binding. Dcx specifically enhances binding of the ADP-bound Kif1a motor domain to MTs. Cryo-electron microscopy and subnanometer-resolution image reconstruction reveal the kinesin-dependent conformational variability of MT-bound Dcx and suggest a model for MAP-motor crosstalk on MTs. Alteration of kinesin run length by MAPs represents a previously undiscovered mode of control of kinesin transport and provides a mechanism for regulation of MT-based transport by local signals.

Articles - 4atx mentioned but not cited (2)

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Reviews citing this publication (26)

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Articles citing this publication (52)

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  37. Different Doublecortin (DCX) Patient Alleles Show Distinct Phenotypes in Cultured Neurons: EVIDENCE FOR DIVERGENT LOSS-OF-FUNCTION AND "OFF-PATHWAY" CELLULAR MECHANISMS. Yap CC, Digilio L, McMahon L, Roszkowska M, Bott CJ, Kruczek K, Winckler B. J Biol Chem 291 26613-26626 (2016)
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  43. Doublecortin facilitates the elongation of the somatic Golgi apparatus into proximal dendrites. Li P, Li L, Yu B, Wang X, Wang Q, Lin J, Zheng Y, Zhu J, He M, Xia Z, Tu M, Liu JS, Lin Z, Fu X. Mol Biol Cell 32 422-434 (2021)
  44. Doublecortin-like kinase 1 compromises DNA repair and induces chromosomal instability. Lu Y, Maruyama J, Kuwata K, Fukuda H, Iwasa H, Arimoto-Matsuzaki K, Sugimura H, Hata Y. Biochem Biophys Rep 16 130-137 (2018)
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  47. Doublecortin-like expressing astrocytes of the suprachiasmatic nucleus are implicated in the biosynthesis of vasopressin and influences circadian rhythms. Coomans C, Saaltink DJ, Deboer T, Tersteeg M, Lanooij S, Schneider AF, Mulder A, van Minnen J, Jost C, Koster AJ, Vreugdenhil E. Glia 69 2752-2766 (2021)
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  49. Doublecortin engages the microtubule lattice through a cooperative binding mode involving its C-terminal domain. Rafiei A, Cruz Tetlalmatzi S, Edrington CH, Lee L, Crowder DA, Saltzberg DJ, Sali A, Brouhard G, Schriemer DC. Elife 11 e66975 (2022)
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  51. Molecular Mechanism of Mutational Disruption of DCLK1 Autoinhibition Provides a Rationale for Inhibitor Screening. Chen W, Liu R, Yu Y, Wei D, Chen Q, Xu Q. Int J Mol Sci 24 14020 (2023)
  52. [JNK/c-Jun signaling pathway mediates arginine vasopressin neuron regeneration by promoting cytoskeleton reconstruction in rats with electrical lesions of the pituitary stalk]. Li K, Feng Z, Ou Y, Zhou M, Peng J, Gong H, Wu G, Liu Y, Qi S. Nan Fang Yi Ke Da Xue Xue Bao 39 1099-1106 (2019)


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