› 4au1

X-ray diffraction
1.45Å resolution

Crystal Structure of CobH (precorrin-8x methyl mutase) complexed with C5 desmethyl-HBA

Released:
DOI: 10.2210/pdb4au1/pdb
PDB entry 4au1 coloured by chain, front view.
PDB entry 4au1 coloured by chain, side view.
PDB entry 4au1 coloured by chain, top view.
Source organism: Rhodobacter capsulatus SB 1003
Primary publication:
An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis.
Deery E, Schroeder S, Lawrence AD, Taylor SL, Seyedarabi A, Waterman J, Wilson KS, Brown D, Geeves MA, Howard MJ, Pickersgill RW, Warren MJ
Nat Chem Biol 8 933-40 (2012)
PMID: 23042036

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cobalamin biosynthesis precorrin-8X methylmutase CobH/CbiC domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 24.19 KDa
Source organism: Rhodobacter capsulatus SB 1003
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D5AV08 (Residues: 1-209; Coverage: 100%)
Gene names: RCAP_rcc02046, cobH
Sequence domains: Precorrin-8X methylmutase
Structure domains: Cobalamin biosynthesis CobH/CbiC, precorrin-8X methylmutase

Ligands and Environments

2 bound ligands:
Ligand P8X 1 x P8X
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 70.55Å b: 65.71Å c: 48.05Å
α: 90° β: 99.32° γ: 90°
R-values:
R R work R free
0.161 0.16 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Microbial production of vitamin B12: a review and future perspectives.
Fang et al. (2017)
6 more

2 mentions without citation

An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis.
Deery et al. (2012)
1 more