› 4awh

X-ray diffraction
2.05Å resolution

Influenza strain pH1N1 2009 polymerase subunit PA endonuclease in complex with rUMP

Released:
DOI: 10.2210/pdb4awh/pdb
PDB entry 4awh coloured by chain, front view.
PDB entry 4awh coloured by chain, side view.
PDB entry 4awh coloured by chain, top view.
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Primary publication:
Structural analysis of specific metal chelating inhibitor binding to the endonuclease domain of influenza pH1N1 (2009) polymerase.
Kowalinski E, Zubieta C, Wolkerstorfer A, Szolar OH, Ruigrok RW, Cusack S
OpenAccess logo PLoS Pathog 8 e1002831 (2012)
PMID: 22876177

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 204 amino acids
Theoretical weight: 23.94 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3W5S0 (Residues: 1-198; Coverage: 28%)
Gene name: PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

2 bound ligands:
Ligand MN 8 x MN
Ligand U5P 4 x U5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 54.94Å b: 120.11Å c: 128.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Influenza virus RNA polymerase: insights into the mechanisms of viral RNA synthesis.
Te Velthuis et al. (2016)
23 more

3 mentions without citation

The Influenza Virus Polymerase Complex: An Update on Its Structure, Functions, and Significance for Antiviral Drug Design.
Stevaert et al. (2016)
2 more