4awl

X-ray diffraction
3.08Å resolution

The NF-Y transcription factor is structurally and functionally a sequence specific histone

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-117225 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Nuclear transcription factor Y subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 9.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23511 (Residues: 262-332; Coverage: 21%)
Gene name: NFYA
Sequence domains: CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Nuclear transcription factor Y subunit beta Chain: B
Molecule details ›
Chain: B
Length: 94 amino acids
Theoretical weight: 10.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25208 (Residues: 51-143; Coverage: 45%)
Gene names: HAP3, NFYB
Sequence domains: Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Structure domains: Histone, subunit A
Nuclear transcription factor Y subunit gamma Chain: C
HSP70 PROMOTER FRAGMENT Chain: I
HSP70 PROMOTER FRAGMENT Chain: J

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 54.7Å b: 62.56Å c: 139.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.25
Expression system: Escherichia coli