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Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands.

Elegheert J, Brigé A, Van Beeumen J, Savvides SN
FEBS Lett 591 3391-3401 (2017)
Related entries: 5k0r, 5k1k, 5k1m, 5k1q, 5k1u, 5k1w

Cited: 2 times
EuropePMC logo PMID: 28869767

Abstract

Shewanella oneidensis, a Gram-negative γ-proteobacterium with an extensive redox capacity, possesses four old yellow enzyme (OYE) homologs. Of these, Shewanella yellow enzyme 4 (SYE4) is implicated in resistance to oxidative stress. Here, we present a series of high-resolution crystal structures for SYE4 in the oxidized and reduced states, and in complex with phenolic ligands and the nitro-aromatic explosive picric acid. The structures unmask new features, including the identification of a binding platform for long-chain hydrophobic molecules. Furthermore, we present the first structural observation of a hydride-Meisenheimer complex of picric acid with a flavoenzyme. Overall, our study exposes the binding promiscuity of SYE4 toward a variety of electrophilic substrates and is consistent with a general detoxification function for SYE4.

Reviews citing this publication (1)

  1. The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453-An Episodic History and Still Mysterious after 60 Years. Willetts A. Microorganisms 9 2593 (2021)

Articles citing this publication (1)

  1. Chlamydomonas reinhardtii mutants deficient for Old Yellow Enzyme 3 exhibit increased photooxidative stress. Böhmer S, Marx C, Goss R, Gilbert M, Sasso S, Happe T, Hemschemeier A. Plant Direct 7 e480 (2023)


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