› 4b60

X-ray diffraction
1.83Å resolution

Structure of rFnBPA(189-505) in complex with fibrinogen gamma chain C- terminal peptide

Released:
DOI: 10.2210/pdb4b60/pdb
PDB entry 4b60 coloured by chain, front view.
PDB entry 4b60 coloured by chain, side view.
PDB entry 4b60 coloured by chain, top view.
Source organisms:
Primary publication:
Evidence for steric regulation of fibrinogen binding to Staphylococcus aureus fibronectin-binding protein A (FnBPA).
Stemberk V, Jones RP, Moroz O, Atkin KE, Edwards AM, Turkenburg JP, Leech AP, Massey RC, Potts JR
OpenAccess logo J Biol Chem 289 12842-51 (2014)
PMID: 24627488

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-526795 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fibronectin-binding protein A Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 35.76 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14738 (Residues: 189-505; Coverage: 32%)
Gene names: SAOUHSC_02803, fnbA
Sequence domains:
Structure domains:
Fibrinogen gamma chain Chains: C, D
Molecule details ›
Chains: C, D
Length: 17 amino acids
Theoretical weight: 1.69 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P02679 (Residues: 421-433; Coverage: 3%)
  • Best match: P02679-2 (Residues: 421-437)
Gene names: FGG, PRO2061

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 37.51Å b: 59.05Å c: 73.49Å
α: 91.86° β: 98.05° γ: 97.87°
R-values:
R R work R free
0.201 0.198 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Staphylococcus aureus Aggregation and Coagulation Mechanisms, and Their Function in Host-Pathogen Interactions.
Crosby et al. (2016)
4 more

5 mentions without citation

Evidence for steric regulation of fibrinogen binding to Staphylococcus aureus fibronectin-binding protein A (FnBPA).
Stemberk et al. (2014)
4 more