› 4bbr

X-ray diffraction
3.4Å resolution

Structure of RNA polymerase II-TFIIB complex

Released:
DOI: 10.2210/pdb4bbr/pdb
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PDB entry 4bbr coloured by chain, front view.
PDB entry 4bbr coloured by chain, side view.
PDB entry 4bbr coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and function of the initially transcribing RNA polymerase II-TFIIB complex.
Sainsbury S, Niesser J, Cramer P
Nature 493 437-40 (2013)
PMID: 23151482

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137345 (preferred)
Entry contents:
13 distinct polypeptide molecules
Macromolecules (13 distinct):
DNA-directed RNA polymerase II subunit RPB1 Chain: A
Molecule details ›
Chain: A
Length: 1733 amino acids
Theoretical weight: 191.82 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P04050 (Residues: 1-1733; Coverage: 100%)
Gene names: D2150, RPB1, RPB220, RPO21, SUA8, YDL140C
Sequence domains:
DNA-directed RNA polymerase II subunit RPB2 Chain: B
Molecule details ›
Chain: B
Length: 1224 amino acids
Theoretical weight: 138.94 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P08518 (Residues: 1-1224; Coverage: 100%)
Gene names: RPB150, RPB2, RPO22, YOR151C
Sequence domains:
Structure domains:
DNA-directed RNA polymerase II subunit RPB3 Chain: C
DNA-directed RNA polymerase II subunit RPB4 Chain: D
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerase II subunit RPB7 Chain: G
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase II subunit RPB11 Chain: K
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
Transcription initiation factor IIB Chain: M

Ligands and Environments

2 bound ligands:
Ligand ZN 9 x ZN
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 222.44Å b: 386.76Å c: 254.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.211
Expression system: Escherichia coli

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Citations

33 review citations

Control of transcriptional elongation.
Kwak et al. (2013)
32 more

11 mentions without citation

Structural visualization of key steps in human transcription initiation.
He et al. (2013)
10 more

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