› 4be7

X-ray diffraction
2.74Å resolution

MUTANT (K220R) OF THE HSDR SUBUNIT OF THE ECOR124I RESTRICTION ENZYME IN COMPLEX WITH ATP

Released:
DOI: 10.2210/pdb4be7/pdb
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PDB entry 4be7 coloured by chain, front view.
PDB entry 4be7 coloured by chain, side view.
PDB entry 4be7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Functional coupling of duplex translocation to DNA cleavage in a type I restriction enzyme.
Csefalvay E, Lapkouski M, Guzanova A, Csefalvay L, Baikova T, Shevelev I, Bialevich V, Shamayeva K, Janscak P, Kuta Smatanova I, Panjikar S, Carey J, Weiserova M, Ettrich R
OpenAccess logo PLoS One 10 e0128700 (2015)
PMID: 26039067

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit Chains: B, D
Molecule details ›
Chains: B, D
Length: 1038 amino acids
Theoretical weight: 120.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10486 (Residues: 1-1038; Coverage: 100%)
Gene names: hsdR, hsr
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 2 x ATP
Ligand PO4 4 x PO4
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 87.045Å b: 124.354Å c: 128.012Å
α: 90° β: 108.86° γ: 90°
R-values:
R R work R free
0.252 0.25 0.292
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

A model for the evolution of prokaryotic DNA restriction-modification systems based upon the structural malleability of Type I restriction-modification enzymes.
Bower et al. (2018)
2 more

3 mentions without citation

Crystal structure of a novel domain of the motor subunit of the Type I restriction enzyme EcoR124 involved in complex assembly and DNA binding.
Grinkevich et al. (2018)
2 more

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