› 4bh5

X-ray diffraction
1.57Å resolution

LytM domain of EnvC, an activator of cell wall amidases in Escherichia coli

Released:
DOI: 10.2210/pdb4bh5/pdb
PDB entry 4bh5 coloured by chain, front view.
PDB entry 4bh5 coloured by chain, side view.
PDB entry 4bh5 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure-function analysis of the LytM domain of EnvC, an activator of cell wall remodelling at the Escherichia coli division site.
Peters NT, Morlot C, Yang DC, Uehara T, Vernet T, Bernhardt TG
Mol Microbiol 89 690-701 (2013)
PMID: 23796240

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Murein hydrolase activator EnvC Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 142 amino acids
Theoretical weight: 15.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37690 (Residues: 278-419; Coverage: 37%)
Gene names: JW5646, b3613, envC, yibP
Sequence domains: Peptidase family M23
Structure domains: Glucose Permease (Domain IIA)

Ligands and Environments

4 bound ligands:
Ligand IOD 23 x IOD
Ligand CL 4 x CL
Ligand GOL 2 x GOL
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P31
Unit cell:
a: 57.404Å b: 57.404Å c: 129.32Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.166 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Regulation of peptidoglycan synthesis and remodelling.
Egan et al. (2020)
3 more

4 mentions without citation

Structure-function analysis of the LytM domain of EnvC, an activator of cell wall remodelling at the Escherichia coli division site.
Peters et al. (2013)
3 more