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Crystal Structure of the Aquifex Reactor Complex Formed by Dihydroorotase (H180A, H232A) with Dihydroorotate and Aspartate Transcarbamoylase with N-(phosphonacetyl)-L-aspartate (PALA)

Source organism: Aquifex aeolicus
Biochemical function: carboxyl- or carbamoyltransferase activity
Biological process: 'de novo' UMP biosynthetic process
Cellular component: cytoplasm

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EC 2.1.3.2: Aspartate carbamoyltransferase

Reaction catalysed:
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Systematic name:
Carbamoyl-phosphate:L-aspartate carbamoyltransferase
Alternative Name(s):
  • ATCase
  • Aspartate carbamyltransferase
  • Aspartate transcarbamoylase
  • Aspartate transcarbamylase
  • Aspartic acid transcarbamoylase
  • Aspartic carbamyltransferase
  • Aspartic transcarbamylase
  • Carbamoylaspartotranskinase
  • Carbamylaspartotranskinase
  • L-aspartate transcarbamoylase
  • L-aspartate transcarbamylase

EC 3.5.2.3: Dihydroorotase

Reaction catalysed:
(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate
Systematic name:
(S)-dihydroorotate amidohydrolase
Alternative Name(s):
  • Carbamoylaspartic dehydrase
  • DHOase
  • Dihydroorotate hydrolase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF00185
Domain description: Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain
Occurring in:
  1. Aspartate carbamoyltransferase catalytic subunit
The deposited structure of PDB entry 4bjh contains 1 copy of Pfam domain PF00185 (Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain) in Aspartate carbamoyltransferase catalytic subunit. Showing 1 copy in chain B.

PF02729
Domain description: Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain
Occurring in:
  1. Aspartate carbamoyltransferase catalytic subunit
The deposited structure of PDB entry 4bjh contains 1 copy of Pfam domain PF02729 (Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain) in Aspartate carbamoyltransferase catalytic subunit. Showing 1 copy in chain B.

PF01979
Domain description: Amidohydrolase family
Occurring in:
  1. Dihydroorotase
The deposited structure of PDB entry 4bjh contains 1 copy of Pfam domain PF01979 (Amidohydrolase family) in Dihydroorotase. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR006130
Domain description: Aspartate/ornithine carbamoyltransferase
Occurring in:
  1. Aspartate carbamoyltransferase catalytic subunit
IPR036901
Domain description: Aspartate/ornithine carbamoyltransferase superfamily
Occurring in:
  1. Aspartate carbamoyltransferase catalytic subunit
IPR002195
Domain description: Dihydroorotase, conserved site
Occurring in:
  1. Dihydroorotase
IPR004722
Domain description: Dihydroorotase
Occurring in:
  1. Dihydroorotase
IPR011059
Domain description: Metal-dependent hydrolase, composite domain superfamily
Occurring in:
  1. Dihydroorotase
IPR006131
Domain description: Aspartate/ornithine carbamoyltransferase, Asp/Orn-binding domain
Occurring in:
  1. Aspartate carbamoyltransferase catalytic subunit
IPR006132
Domain description: Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding
Occurring in:
  1. Aspartate carbamoyltransferase catalytic subunit
IPR002082
Domain description: Aspartate carbamoyltransferase
Occurring in:
  1. Aspartate carbamoyltransferase catalytic subunit
IPR032466
Domain description: Metal-dependent hydrolase
Occurring in:
  1. Dihydroorotase
IPR006680
Domain description: Amidohydrolase-related
Occurring in:
  1. Dihydroorotase
IPR009017
Domain description: Green fluorescent protein
Occurring in:
  1. Dihydroorotase

Structure domains

CATH CATH domains
3.40.50.1370
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: Aspartate/ornithine carbamoyltransferase
Occurring in:
  1. Aspartate carbamoyltransferase catalytic subunit
The deposited structure of PDB entry 4bjh contains 2 copies of CATH domain 3.40.50.1370 (Rossmann fold) in Aspartate carbamoyltransferase catalytic subunit. Showing 2 copies in chain B.
2.30.40.10
Class: Mainly Beta
Architecture: Roll
Topology: Urease, subunit C; domain 1
Homology: Urease, subunit C, domain 1
Occurring in:
  1. Dihydroorotase
The deposited structure of PDB entry 4bjh contains 1 copy of CATH domain 2.30.40.10 (Urease, subunit C; domain 1) in Dihydroorotase. Showing 1 copy in chain A.
3.20.20.140
Class: Alpha Beta
Architecture: Alpha-Beta Barrel
Topology: TIM Barrel
Homology: Metal-dependent hydrolases
Occurring in:
  1. Dihydroorotase
The deposited structure of PDB entry 4bjh contains 1 copy of CATH domain 3.20.20.140 (TIM Barrel) in Dihydroorotase. Showing 1 copy in chain A.

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