› 4bnd

X-ray diffraction
1.5Å resolution

Structure of an atypical alpha-phosphoglucomutase similar to eukaryotic phosphomannomutases

Released:
DOI: 10.2210/pdb4bnd/pdb
PDB entry 4bnd coloured by chain, front view.
PDB entry 4bnd coloured by chain, side view.
PDB entry 4bnd coloured by chain, top view.
Source organism: Lactococcus cremoris subsp. cremoris MG1363
Primary publication:
High-resolution structure of an atypical α-phosphoglucomutase related to eukaryotic phosphomannomutases.
Nogly P, Matias PM, de Rosa M, Castro R, Santos H, Neves AR, Archer M
Acta Crystallogr D Biol Crystallogr 69 2008-16 (2013)
PMID: 24100319

Function and Biology Details

Reaction catalysed: 
Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106938 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
phosphomannomutase Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.43 KDa
Source organism: Lactococcus cremoris subsp. cremoris MG1363
Expression system: Lactococcus lactis subsp. cremoris NZ9000
UniProt:
  • Canonical: A2RIP9 (Residues: 1-252; Coverage: 100%)
Gene names: llmg_0541, pgmA
Sequence domains: Eukaryotic phosphomannomutase
Structure domains:
phosphomannomutase Chain: B
Molecule details ›
Chain: B
Length: 253 amino acids
Theoretical weight: 28.37 KDa
Source organism: Lactococcus cremoris subsp. cremoris MG1363
Expression system: Lactococcus lactis subsp. cremoris NZ9000
UniProt:
  • Canonical: A2RIP9 (Residues: 1-252; Coverage: 100%)
Gene names: llmg_0541, pgmA
Sequence domains: Eukaryotic phosphomannomutase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3121
Unit cell:
a: 67.174Å b: 67.174Å c: 210.394Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.156 0.18
Expression system: Lactococcus lactis subsp. cremoris NZ9000

Quick links

Citations

2 mentions without citation

Conformational response to ligand binding in phosphomannomutase2: insights into inborn glycosylation disorder.
Andreotti et al. (2014)
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